Beilstein J. Org. Chem.2019,15, 2184–2190, doi:10.3762/bjoc.15.215
synthesised in amorphadienesynthase-catalysed reactions from 8- and 12-methoxyfarnesyl diphosphates due to the highly plastic yet tightly controlled carbocationic chemistry of this sesquiterpene cyclase.
Keywords: artemisinin; amorphadienesynthase; oxygenated terpenoids; sesquiterpenoids; substrate
engineering; terpenes; Introduction
Amorphadienesynthase (ADS) from Artemisia annua is a key enzyme involved in the biosynthesis of the antimalarial sesquiterpene drug artemisinin (1) [1][2][3][4]. ADS catalyses the Mg2+-dependent conversion of farnesyl diphosphate (FDP, 2) to amorpha-4,11-diene (3) with
deprotonation from C15 and C4 from intermediate 30 affords 12-methoxy-β-sesquiphellandrene (26) and 12-methoxyzingiberene (27), respectively (Scheme 4).
Conclusion
In conclusion, the class I sesquiterpene cyclase amorphadienesynthase facilitates the efficient conversion of readily accessible synthetic methoxy
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Graphical Abstract
Scheme 1:
Mechanism of the ADS-catalysed conversion of FDP (2) to amorpha-4,11-diene (3), a biosynthetic prec...